A detailed explanation of the PGTseq-M method and its requirements is included in the Juno Genetics Instruction Manual. In summary, Juno will start working on a new PGTseq-M test (prePGT-M) once the following is received: 1) genetic reports describing the mutation(s) responsible for the disorder(s) for which PGTseq-M is requested; 2) blood samples from the couple requesting PGTseq-M (both male and female, even if only one of them is carrier of the mutation); 3) whenever possible, a blood, DNA or saliva sample from a child or the patient’s mother/father tested for the mutation; 4) a completed test request form for PGTseq-M which includes relevant patient information, including genetic information. A prePGT-M test requires several weeks to be developed in each case. Although in most cases, the prePGT-M will have a successful outcome, in rare cases it will be technically not feasible. For these reasons, patients should not start an IVF cycle until Juno has confirmed that the prePGT-M test has been successfully developed.