OUR TESTS

PGT[A]Seq

Increases the chance of a healthy birth per embryo transfer

Best-in-class accuracy

A high number of euploid embryos reported

Improved clinical outcomes

Fewer embryos classified mosaic

The best-in-class accuracy of PGT[A]Seq means an increased number of euploid embryos are correctly reported, leading to more viable embryos being transferred with higher pregnancy rates than is achieved using less accurate PGT-A methods.

More embryos are reported ‘euploid’ using PGT[A]Seq compared to some other PGT-A methods

Advanced next generation sequencing method

Unlike most other methods, PGT[A]Seq combines two distinct pieces of information to provide unparalleled accuracy. The test simultaneously assesses the quantity of DNA at thousands of sites on each chromosome using an advanced next generation sequencing method. The test also evaluates numerous variations in the DNA sequence, known as single nucleotide polymorphisms (SNPs). Together, these two pieces of information provide major advantages over other PGT-A methods, including the detection of triploid embryos. The PGT[A]Seq technology is the most accurate test of its kind, with proven clinical validity.

– The most powerful embryo selection tool

– The most accurate and highly validated test of its kind

PGT[A]Seq method provides two pieces of information:

NGS

Quantity of DNA measured at thousands of individual sites

SNPs

Genotypes determined for thousands of DNA polymorphisms

Clinical impact

– Avoid unsuccessful transfer of non-viable aneuploid embryos

– Reduced miscarriage rate

– Reduced risk of aneuploid syndromes

– Faster time to pregnancy

– Permits high efficiency single embryo transfer (SET)

– Accurate detection of triploidy

– Avoids incorrect ‘mosaic’ designation

– Higher proportion of embryos classified ‘euploid’

PGT[A]Seq by Juno

The most powerful and accurate embryo analysis on the market

Play Video

Juno uses a unique PGT[A]Seq methodology, which has successfully demonstrated clinical validity in a non-selectin study

Our PGT[A]Seq technique employs the latest next-generation sequencing methods, increasing the quality of the data obtained and yielding results of unparalleled accuracy

How does it work?

01

DNA amplified from all 24 chromosomes

02

Matching fragments of DNA with the chromosomes they came from

03

Measurement of the quantity of DNA for each chromosome

1

NGS

Juno uses next-generation sequencing to measure the amount of DNA at thousands of sites on each chromosome. This allows the number of copies of the chromosome to be calculated with high accuracy.

2

SNPs

Juno looks at thousands of places where the DNA sequence can differ between individual chromosomes. Each of these sites of variation can be type ’A’ or type ‘B’. Normal, Trisomy and monosomy each have characteristic patterns of As and Bs.

Together, the measurement of the amount of DNA and the analysis of the DNA sequence greatly increases the accuracy of PGT[A]Seq

We love embryos

Juno PGT[A]Seq increases the chance of a healthy birth per embryo transfer

PGT[A]Seq

Process

STEP 1

Biopsy and embryo vitrification

STEP 2

PGT[A]Seq

STEP 3

RESULTS
7 working days

STEP 4

Transfer of normal frozen embryos

Juno

vs

other labs

Other labs

JUNO

25,007 embryos analyzed.

%transferable per informative embryo

REDUCTION IN

MOSAIC

EMBRYOS

The enhanced accuracy of PGT[A]Seq prevents viable embryos from being incorrectly classified mosaic.

MORE EUPLOID EMBRYOS
0 %
EXTRA EMBRYOS reported for transfer from a dataset of 25,007 embryos derived from 15 different IVF clinics
+ 0
EMBRYOS AVAILABLE to Transfer
+ 0

Know your
Clinical Rates

Improved clinical outcomes for cycles using preimplantation genetic testing for aneuploidy (PGT-A) may be associated with a change in genetic service provider

Other labs

JUNO

25,007 embryos analyzed.

%transferable per informative embryo

Background

An increasing number of IVF cycles include PGT-A to assist in the selection of euploid embryos for transfer to the uterus. All modern PGT laboratories utilise next generation sequencing (NGS) to predict the copy number of each chromosome. Given this technical convergence, it has generally been supposed that the choice of PGT-A provider need only depend on factors such as the quality of the user experience, convenience and price. Here we consider whether the choice of PGT-A provider might have more profound affects, potentially impacting clinical results.

Methods

A large network of IVF clinics switched from PGT-A provider ‘A’ to provider ‘B’. The final 6 months of clinical data using A was compared to 6 months of data after the switch to B. No significant changes in any aspect of patient population, treatment or embryological practice occurred between the two time periods evaluated.

Results

Within the two time periods considered, 9,091 embryos underwent PGT-A using A and 9,550 using B. The average female age was 39.0 and 39.3, respectively. Differences in important clinical outcomes were observed, which were particularly apparent for female patients <38 years of age. For that group, A versus B results were: 52.5% of embryos classified euploid vs. 57.8% (p<0.0001); 45% ongoing pregnancy after the first embryo transfer vs. 53% (p=0.04); 18% miscarriage rate vs. 11% (p=0.048).

Conclusions

PGT-A and NGS are umbrella terms encompassing different methods with widely varying levels of validation and accuracy. The results of this study suggest that the choice of PGT-A provider has implications for clinical results. One possible interpretation of the data is that higher rates of euploidy and pregnancy with B might be a consequence of embryos being wrongly classified as aneuploid by A, while incorrect labelling of abnormal embryos as euploid could explain the relative increase in miscarriage rates when using that provider.

PGT-A Seq Method

Juno vs other labs

Independent study conducted by Bardos et al (ASRM, 2021), comparing four PGT-A providers (one of which was Juno). The study, involving oocyte donation cycles, confirms that Juno classifies 10-20% more embryos euploid in young patients. Birth rates appear higher for Juno cycles (6-10%), although the study was not powered to look at that outcome.

REDUCTION IN
MOSAIC EMBRYOS

The enhanced accuracy of PGT[A]Seq prevents viable embryos from being incorrectly classified mosaic.

ONGOING PREGNANCIES
0 ,00%
MORE EUPLOID EMBRYOS REPORTED
0 ,30%
EMBRYOS AVAILABLE TO TRANSFER
+ 0 ,30%

Advantages of using the Juno PGT[A]Seq

01
The most powerful embryo selection tool currently available
02
High accuracy, including detection of triploid embryos and detection of DNA contamination
03
Avoid unsuccessful transfer of non-viable aneuploid embryos
04
Permits high efficiency single embryo transfer (SET)
05
Faster time to pregnancy
06
Provides information on likely potential of stored material and avoids storage of non-viable embryos
07
Reduced miscarriage rate
08
Reduced risk of aneuploid syndromes
09
Avoids incorrect classification of euploid embryos as abnormal or mosaic
10
Compared to other methods, Juno PGT[A]Seq is associated with a higher proportion of embryos categorised as euploid

Who should use PGT[A]Seq?

All couples undergoing IVF have a high likelihood that some of their embryos will be aneuploid. Such embryos typically fail to implant, miscarry, or more rarely produce abnormal pregnancies affected by problems such as Down syndrome. Consequently, PGT[A]Seq is likely to have value for all couples. However, testing chromosomes in embryos becomes progressively more important as women age. By the age of 35 it is typical for more than one-third of embryos to be aneuploid, and by the time a woman is over 40, more than three-quarters of embryos are chromosomally abnormal.

Chromosome abnormalities are invisible without using specially designed tests. PGT-A[seq] is the most accurate method for distinguishing embryos that have the potential to form a healthy pregnancy from embryos that are not viable due to aneuploidy. Identifying the embryos that are most likely to produce a pregnancy, and ensuring they are prioritized for transfer to the uterus, helps maintain the highest possible pregnancy rates when using single embryo transfer (SET) strategies to reduce the incidence of multiple gestations.

The time required for the shipment of the samples, and for the complex laboratory procedures, means that the embryos must be cryopreserved (vitrified) while the test is being performed. The embryos can usually be thawed and transferred during the next cycle.

PGT[A]Seq results are typically available 6 days after Juno receives the embryo biopsy samples (although results can take up to 10 days).

Our goal is to make working with Juno Genetics as easy as possible. Juno is committed to support the clinics we work with by providing guidance at all stages of the PGT process. We are available to help with queries and provide advice. At Juno we understand different clinics may have unique needs and our policy is based on flexibility, where we adapt to the specific requirements of each clinic. If your clinic already offers PGT in collaboration with another genetic laboratory, then it is likely that very few, if any, significant changes will be necessary. Please contact Juno Genetics and we will be happy to guide you through the registration procedure, providing you with all the necessary documentation and solving any questions you may have. If your clinic is new to PGT, Juno can assist by providing information and documentation to help you get started. Juno can also assist with training and evaluation of procedures, ensuring that all elements of the PGT procedure are optimized and functioning properly, prior to launch a clinical service. Please contact supportspain@junogenetics.com to get started with Juno.

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